In this shot (L to R): Centrifuge, Microtubule tray, Pipettes on holder, box of gloves, PCR, Heater pad, Water Bath
Friday, June 19, 2009
Uncluttered Bench - Long view
In this shot (L to R): Centrifuge, Microtubule tray, Pipettes on holder, box of gloves, PCR, Heater pad, Water Bath
PCR - Basic
In the shot: PCR, pipettes, pipette tips (small glass tips), microtubes, microtube trays, latex glovesNote: Generally you would use the smaller pipettes shown as you are working with small quantities.
PCR - Plus
In the shot: PCR machine, pipettes, enterotube, petri dishesIn the lab but not used with PCR: The other items are all reasonable candidates to add to the same bench in this type of lab.
Uses: DNA analysis when you've only got a little blood... You can make lots of copies of a specific piece of DNA.
Labs use Polymerase chain reaction (PCR) machines to detect and amplify a specific DNA sequence against the background of a complex genome. This procedure is very powerful because it can be used on fragments of DNE that are initially present in infinitesimally small quantities.
Tissue Staining - Basic
In the shot: Stain set-up with Gram tissue stain, clear slides, rinse bucket, water bottle, bacterial transfer loop, gloves
A glass slide is placed on the bar across of the stain tray, a dime size drop of dark purple stain is added.
Tissue Staining - The Rinse
In the shot: Lab tech holds up the clear glass slide and rinses the dark purple gram stain off. They would normally be wearing gloves and would rinse the slide multiple times.
Add: latex gloves with fingertips dyed yellow or dark purple.
Pipetting a sample
In this shot: Pipette holder, pipettes (in white covers), pipette and test tube trays
Dissection Scope - Close-up
In the shot: Dissection scope, scalpel, petri dish with bacterial culture.
Dissection Scope - Basic
In the shot: Dissection scope, scalpel, petri dish with bacterial culture. Dissection work is often done near a sink. Options: Add a sink, dish soap and latex gloves nearby. Bits of bone, quarter size piece of tissue being dissected, plant material, larger items to be magnified.
Additional: camel hair brush, morgue (small bottle with water and detergent), culture vials (square bottles), deionized water, label tape, markers, white cards, scissors, anaesthetizing vial (round bottles) add if you are using live insects, etc.
Compound Microscope Tray
In this shot: Compound microscope tray with slides, slide box, spearpoint needle, alcohol dropsAdd: slides and cover slips, water dropping bottle, 4 petri plates with agar, agar bottle, 70% ethanol, alcohol lamp, matches, container for alcohol flaming, spearpoint needle, spatula
Compound Microscope - Basic
In this shot: Compound microscopeAdd: slides and cover slips, water dropping bottle, 4 petri plates with agar, agar bottle, 70% ethanol, alcohol lamp, matches, container for alcohol flaming, spearpoint needle, spatula
Thermal Heater - Basic
In the shot: Heating base, microtubual tray, single microtube.Heat is used in many processes. The rack may contain a single microtube or many. There is no need to balance the tray as the sample sits still and does not spin as in the centrifuge.
CENTREFUGE - UNBALANCED
You always want to keep the centrifuge balanced. There should be two microtubes across from one another (a single orange colored microtube is in view. Having two directly across from one another keeps the centrifuge balanced as it spins.
Electrophoresis - Plus
In this shot: Electrophoresis analytic tool, slides, notebook, gelAdd: Buffer, test tubes, brand name stains like SimplyBlue™ SafeStain and SYPRO® Ruby are used for detection of proteins in gels.
Electrophoresis is an analytical tool for separating proteins and nucleic acids. An electric current is passed through a medium containing the target mixture, and each type of molecule travels through the medium at a different rate, depending on its electrical charge and/or size. Agarose and acrylamide gels are the most common media for electrophoresis of nucleic acids and proteins, respectively.
Biohazard Cabinet
In this shot: Flammable Biohazard Cabinet with shelving above. Shelves contain plastic labware.
FUMEHOOD - AS STORAGE
In the shot: Fumehood being used as storage for extra buckets, etc. Not proper safety protocol, but commonly done.
Petri Dish - E. Coli
In this shot: E.Coli colony. The petri dishes are often coated red or green and change color depending on whether they ferment certain sugars.
Agar
In this photo: bottles of agar, a growth medium added to a petri dish to grow bacterial colonies
Labware Labelling
In this shot: Flask with hand-written label
Hand-labelling is common in labs. Any beaker or flask can be labelled with red, blue, orange, pink, yellow or white tape.
Common labels clear liquids: 0.1 gr/1000 ml TETRA lolium (toxic)
H2So4, CH4SO4, Acidified H2O2, 0.0125 M HCL
Common labels on Brown (liteSafe) bottles:
Iodine, Carolina BLU DNA Stain, Phosphoric Acid (85%)
Laboratory Chemicals
In this shot: Iodine and other light sensitive chemicals in brown bottles.Common labels on Brown (light free) bottles:
Iodine, Carolina BLU DNA Stain, Phosphoric Acid (85%)
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